Blar i forfatter "Smedsrød, Bård"

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    • 14th International Symposium on Cells of the Hepatic Sinusoid 

      Smedsrød, Bård (Book; Bok, 2008-08-31)
      Abstract book of the symposium

    • Asialoglycoprotein Receptor (ASGP-R) is Liver Parenchymal Cells is Involved in Elimination of Recombinant Human TFPI 

      Appa, Rupa Shree; Øie, Cristina Ionica; Brodin, Ellen; Hansen, John-Bjarne; Smedsrød, Bård (Journal article; Tidsskriftartikkel, 2010)
      We here report on a study carried out to determine the early clearance kinetics, and organ, cell(s) and receptor(s) responsible for the clearance of full length TFPI purified from BHK cells (TFPI<sup>BHK</sup>). Following intravenous administration, <sup>125</sup>I-TFPI<sup>BHK</sup> was cleared with a biphasic elimination curve, and with a significantly slower t<sub>1/2</sub>α compared to recombinant ...

    • Autofluorescence in freshly isolated adult human liver sinusoidal cells 

      Larsen, Anett Kristin; Simon-Santamaria, Jaione; Elvevold, Kjetil; Ericzon, Bo Gøran; Mortensen, Kim Erlend; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-13)
      Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment ...

    • Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone 

      Li, Ruomei; Bhandari, Sabin; Martinez-Zubiaurre, Inigo; Bruun, Jack-Ansgar; Urbarova, Ilona Halva; Smedsrød, Bård; Simon-Santamaria, Jaione; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-02)
      Introduction<p> <p>Liver sinusoidal endothelial cells (LSECs) are specialized fenestrated scavenger endothelial cells involved in the elimination of modified plasma proteins and tissue turnover waste macromolecules from blood. LSECs also participate in liver immune responses. A challenge when studying LSEC biology is the rapid loss of the in vivo phenotype in culture. In this study, we have ...

    • Delivery is key: lessons learnt from developing splice-switching antisense therapies 

      Godfrey, Caroline; Desviat, Lourdes R.; Smedsrød, Bård; Piétri-Rouxel, France; Denti, Michela A.; Disterer, Petra; Lorain, Stéphanie; Nogales-Gadea, Gisela; Sardone, Valentina; Anwar, Rayan; El Andaloussi, Samir; Letho, Taavi; Khoo, Bernard; Brolin, Camilla; Mc Van Roon-Mom, Willeke; Goyenvalle, Aurélie; Aartsma-Rus, Annemieke; Arechavala-Gomeza, Virginia (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-03-13)
      The use of splice-switching antisense therapy is highly promising, with a wealth of pre-clinical data and numerous clinical trials ongoing. Nevertheless, its potential to treat a variety of disorders has yet to be realized. The main obstacle impeding the clinical translation of this approach is the relatively poor delivery of anti- sense oligonucleotides to target tissues ...

    • Effect of Unfractionated Heparin on TFPI Elimination 

      Øie, Cristina Ionica; Appa, Rupa Shree; Brodin, Ellen; Hilden, Ida; Smedsrød, Bård; Hansen, John-Bjarne (Journal article; Tidsskriftartikkel, 2010)
      Background: Tissue factor pathway inhibitor (TFPI) plays an important role for the anticoagulant effect of heparin. Depletion of intravascular TFPI by treatment with unfractionated heparin (UFH), and not by low molecular weight heparin (LMWH), has been suggested to explain the superiority of LMWH in treatment of both arterial and venous thrombosis. The present study was undertaken to investigate ...

    • Efficient uptake of blood-borne BK and JC polyomavirus-like particles in endothelial cells of liver sinusoids and renal vasa recta 

      Simon-Santamaria, Jaione; Rinaldo, Christine Hanssen; Kardas, Piotr; Li, Ruomei; Malovic, Ivana; Elvevold, Kjetil H.; McCourt, Peter Anthony; Smedsrød, Bård; Hirsch, Hans H.; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-11-06)

    • FITC conjugation markedly enhances hepatic clearance of N-formyl peptides 

      Øie, Cristina Ionica; Snapkov, Igor; Elvevold, Kjetil; Sveinbjørnsson, Baldur; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-08-05)
      In both septic and aseptic inflammation, N-formyl peptides may enter the circulation and induce a systemic inflammatory response syndrome similar to that observed during septic shock. The inflammatory response is brought about by the binding of N-formyl peptide to formyl peptide receptors (FPRs), specific signaling receptors expressed on myeloid as well as non-myeloid cells involved in the ...

    • Genome-wide analysis of DNA methylation and gene Expression patterns in purified, uncultures human liver cells and activated hepatic stellate cells 

      Taghdouini, Adil El; Sørensen, Anita Løvstad; Reiner, Andrew Henry; Coll, Mar; Verhulst, Stefaan; Mannaerts, Inge; Øie, Cristina Ionica; Smedsrød, Bård; Najimi, Mustapha; Sokal, Etienne; Luttun, Aernout; Sancho-Bru, Pau; Collas, Philippe; Grunsven, Leo A van (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-08-31)
      <p>Background & Aims: Liver fibrogenesis – scarring of the liver that can lead to cirrhosis and liver cancer – is characterized by hepatocyte impairment, capillarization of liver sinusoidal endothelial cells (LSECs) and hepatic stellate cell (HSC) activation. To date, the molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. Here, we assess the transcriptome ...

    • Hepatic disposal of advanced glycation end products during maturation and aging 

      Svistounov, Dmitri; Oteiza, Ana; Zykova, Svetlana; Sørensen, Karen Kristine; McCourt, Peter Anthony; McLachlan, Andrew J; Mccuskey, Robert Scott; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      Aging is characterized by progressive loss of metabolic and biochemical functions and accumulation of metabolic by-products, including advanced glycation end products (AGEs), which are observed in several pathological conditions. A number of waste macromolecules, including AGEs are taken up from the circulation by endocytosis mainly into liver sinusoidal endothelial cells (LSECs) and Kupffer cells ...

    • High-affinity von Willebrand factor binding does not affect the anatomical or hepatocellular distribution of factor VIII in rats 

      Øie, Cristina Ionica; Roepstorff, Kirstine; Behrens, Carsten; Bøggild Kristensen, Jesper; Karpf, Ditte Maria; Bolt, Gert; Gudme, Charlotte N.; Kjalke, Marianne; Smedsrød, Bård; Appa, Rupa Shree (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-07-05)
      Background: Von Willebrand factor (VWF) stabilizes factor VIII in the circulation and prevents its premature clearance.<p><p> Objective: To study the effects of VWF on FVIII clearance in rats with endogenous VWF.<p><p> Methods: Anatomical and hepatocellular distribution studies were performed in rats following intravenous administration of glycoiodinated recombinant FVIII (rFVIII) and a ...

    • The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells 

      Martinez, Inigo Zubiavrre; Warren, A; Le Couteur, D.G.; Nedredal, Geir Ivar; Øie, Cristina Ionica; Johansen, oddmund; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2008)

    • Liver sinusoidal endothelial cell ICAM-1 mediated tumor/endothelial crosstalk drives the development of liver metastasis by initiating inflammatory and angiogenic responses 

      Benedicto, Aitor; Herrero, Alba; Romayor, Irene; Marquez, Joana; Smedsrød, Bård; Olaso, Elvira; Arteta, Beatriz (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-09-11)
      The prometastatic stroma generated through tumor cells/host cells interaction is critical for metastatic growth. To elucidate the role of ICAM-1 on the crosstalk between tumor and primary liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs), implicated in tumor adhesion and angiogenesis, we performed <i>in vitro</i> cocultures and an <i>in vivo</i> model of liver metastasis ...

    • Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses 

      Øie, Cristina Ionica; Wolfson, Deanna; Yasunori, Tanji; Dumitriu, Gianina; Sørensen, Karen Kristine; McCourt, Peter Anthony; Ahluwalia, Balpreet Singh; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-21)
      The liver is constantly exposed to dietary antigens, viruses, and bacterial products with inflammatory potential. For decades cellular uptake of virus has been studied in connection with infection, while the few studies designed to look into clearance mechanisms focused mainly on the role of macrophages. In recent years, attention has been directed towards the liver sinusoidal endothelial cells ...

    • Liver sinusoidal endothelial cells represents an important blood clearance system in pigs 

      Nedredal, Geir I.; Elvevold, Kjetil H.; Olsen, Randi; Ytrebø, Lars M.; Revhaug, Arthur; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2003-01-03)
      Background: Numerous studies in rats and a few other mammalian species, including man, have shown that the sinusoidal cells constitute an important part of liver function. In the pig, however, which is frequently used in studies on liver transplantation and liver failure models, our knowledge about the function of hepatic sinusoidal cells is scarce. We have explored the scavenger function of pig ...

    • Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIB, yet maintain a preserved fenestration the Glmp gt/gt mouse model of slowly progressing liver fibrosis 

      Antwi, Milton Boaheng; Dumitriu, Gianina; Simón-Santamaria, Jaione; Sánchez Romano, Javier; Li, Ruomei; Smedsrød, Bård; Vik, Anders; Eskild, Winnie; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-11-01)
      Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp<sup>gt/gt</sup> mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born ...

    • Peer reviewing: a private affair between the individual researcher and the publishing houses, or a responsibility of the university? 

      Longva, Leif; Reierth, Eirik; Moksness, Lars; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2017)
      Peer reviewing is mandatory for scientific journals as quality control of submitted manuscripts, for universities to rank applicants for scientific positions, and for funding agencies to rank grant applications. In spite of this deep dependency of peer reviewing throughout the entire academic realm, universities exhibit a peculiar lack of interest in this activity. The aim of this article is to show ...

    • pH-dependent structural transitions in cationic ionizable lipid mesophases are critical for lipid nanoparticle function 

      Philipp, Julian; Dabkowska, Aleksandra; Reiser, Anita; Frank, Kilian; Krzysztoń, Rafał; Brummer, Christiane; Nickel, Bert; Blanchet, Clement E.; Sudarsan, Akhil; Ibrahim, Mohd; Johansson, Svante; Skantze, Pia; Skantze, Urban; Östman, Sofia; Johansson, Marie; Henderson, Neil; Elvevold, Kjetil; Smedsrød, Bård; Schwierz, Nadine; Lindfors, Lennart; Rädler, Joachim O. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-06)
      Lipid nanoparticles (LNPs) are advanced core-shell particles for messenger RNA (mRNA) based therapies that are made of polyethylene glycol (PEG) lipid, distearoylphosphatidylcholine (DSPC), cationic ionizable lipid (CIL), cholesterol (chol), and mRNA. Yet the mechanism of pH-dependent response that is believed to cause endosomal release of LNPs is not well understood. Here, we show that eGFP (enhanced ...

    • Plan S og implementering 

      Longva, Leif; Frantsvåg, Jan Erik; Smedsrød, Bård (Chronicle; Kronikk, 2018-11-05)
      Universitetene bør ta ansvar for å sikre at fagfellevurderingen blir utført på beste måte, skriver forfatterne av dette innlegget.

    • Prediction of human drug-induced liver injury (DILI) in relation to oral doses and blood concentrations 

      Albrecht, Wiebke; Kappenberg, Franziska; Brecklinghaus, Tim; Stoeber, Regina; Marchan, Rosemarie; Zhang, Mian; Ebbert, Kristina; Kirschner, Hendrik; Grinberg, Marianna; Leist, Marcel; Moritz, Wolfgang; Cadenas, Cristina; Ghallab, Ahmed; Reinders, Jörg; Vartak, Nachiket; van Thriel, Christoph; Golka, Klaus; Tolosa, Laia; Castell, José V.; Damm, Georg; Seehofer, Daniel; Lampen, Alfonso; Braeuning, Albert; Buhrke, Thorsten; Behr, Anne-Cathrin; Oberemm, Axel; Gu, Xiaolong; Kittana, Naim; van de Water, Bob; Kreiling, Reinhard; Fayyaz, Susann; van Aerts, Leon; Smedsrød, Bård; Ellinger-Ziegelbauer, Heidrun; Steger-Hartmann, Thomas; Gundert-Remy, Ursula; Zeigerer, Anja; Ullrich, Anett; Runge, Dieter; Lee, Serene M.L.; Schiergens, Tobias S.; Kuepfer, Lars; Aguayo-Orozco, Alejandro; Sachinidis, Agapios; Edlund, Karolina; Gardner, Iain; Rahnenführer, Jörg; Hengstler, Jan G. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-27)
      Drug-induced liver injury (DILI) cannot be accurately predicted by animal models. In addition, currently available in vitro methods do not allow for the estimation of hepatotoxic doses or the determination of an acceptable daily intake (ADI). To overcome this limitation, an in vitro/in silico method was established that predicts the risk of human DILI in relation to oral doses and blood concentrations. ...